11/9/2023 0 Comments Endospore forming bacteria![]() The purpose of this step is to steam the slide and not to overheat it. Keep the blotting paper moist by adding drops of malachite green. ![]() Heat, remove and re-heat the slide for about five minutes.Boiling water or a Bunsen burner can be used to heat the slide. Place the slide on the heat until it evaporates.A blotting paper is put on the slide and the malachite green stain solution is pour on the slide.Smear the sample to be studied at the center of the slide.Distilled water – it acts as the decolorizing agent.The malachite green (0.5 grams) should be dissolved in water (100 ml). Malachite green – it is used as the primary stain.Image 3: An endospore staining procedure using the Schaeffer-Dulton Technique. #1- Schaeffer-Dulton Technique (modified method) The staining method for endospores is done in two ways: Dorner’s Method staining technique (traditional method) and Schaeffer-Fulton staining technique (modified method). They are not linked with vegetative bacteria. Mature endospores are seen as green ellipses too.On the other hand, the spores are viewed as green ellipses inside the cells. The vegetative cells containing endospores should be stained pink.The color of the vegetative cell is pink or reddish.Once you look at the cells under the microscope, you will notice the following: To stain the decolorized vegetative cells, a counterstain should be added. In other words, it has the ability to retain its primary dye even if the vegetative cells will lose the stain. However, once the spore wall is dyed, the color will lock in it.Įndospores are also resistant to de-staining. The dye comes right out of the cell is washed thoroughly. Such a procedure does not require the use of decolorizer because malachite green binds to the cell and spore wall. It uses the heating process to act as a mordant as endospores have the ability to resist staining. (1, 2, 3, and 4)Ī primary stain in the form of malachite green is used for staining endospores. It is also used to differentiate spore-forming bacteria from non-spore forming. Today, the Shaeffer and Fulton method is commonly used to differentiate bacterial endospores from other vegetative cells. The modified process is simpler and faster. In 1933, the procedure was modified by Shaeffer and Fulton. Anthrax is also caused by endospore-forming bacteria.Ī staining method for endospore was published by Dorner in 1922. Some of them can also be the reason for food poisoning. Some can be found in medical settings such as in the case of patients with tetanus, botulism, and gas gangrene. This is their coping mechanism for them to survive even in an unfavorable environment.Įndospore-forming bacteria are usually found in the soil as well as in the aquatic environment. #1- Schaeffer-Dulton Technique (modified method)Īs a result, they produce inactive form-endospore.What are the principles of endospore staining?.What is the significance of endospore staining?.
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